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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Urinalysis and Other Body Fluids | 5–10% | - Physical and chemical testing - Microscopic analysis and disease correlation |
| Topic 2: Microbiology | 15–20% | - Virology and parasitology testing - Bacteriology and mycology procedures |
| Topic 3: Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
| Topic 4: Blood Banking | 15–20% | - Transfusion practice and testing - Blood products and components - Blood group systems and immunology |
| Topic 5: Immunology | 5–10% | - Serologic procedures and immune diseases - Principles of immunology |
| Topic 6: Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Topic 7: Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Clostridium difficile is a gram-positive, anaerobic, spore-forming bacillus that produces a strong toxin. C difficile is a common cause of antibiotic-associated diarrhea and pseudomembranous colitis.
Microbiology
A recto-sigmoidoscopy revealed pseudomembranes in a patient with severe diarrhea following prolonged treatment with ampicillin. Which of the following organisms is MOST likely to be isolated?
- A. Bacteroides fragilis
- B. Clostridium botulinum
- C. Klebsiella oxytoca
- D. Clostridium difficile
- E. Fusobacterium nucleatum
Correct Answer: D 🗳️
Which of the following is NOT part of the stand or framework of the microscope?
- A. arm
- B. stage
- C. base
- D. ocular lenses
Correct Answer: D 🗳️
The Westgard multi-rule 22S describes the scenario where two consecutive data points fall outside +2SD or
-2SD. If this occurs, then the run must be rejected. This situation is most likely caused by a systematic error.
Which of the following describes the Westgard multi-rule 22S?
- A. Two points are within 2SD
- B. Two control data points are within ± 2s
- C. One control data point falls outside +2s and a second point falls outside - 2s
- D. Two consecutive data points fall inside +2s
- E. Two consecutive data points fall outside +2SD or -2SD
Correct Answer: E 🗳️
Polyspecific antihuman globulin (AHG) reagent contains both anti-IgG and anti-C3d.
Polyspecific antihuman globulin (AHG) reagent used in antiglobulin testing should react with which one of the following?
- A. IgG and IgA
- B. IgG and C3d
- C. IgM and C3d
- D. IgM and IgA
Correct Answer: B 🗳️
The part of the microscope that holds the objective lenses is called the _______________.
- A. objective
- B. revolving nosepiece
- C. condenser
- D. fine adjustment
Correct Answer: B 🗳️





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